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Difco™ & BBL™ Manual, 2nd Edition. Spirit Blue Agar. Lipase Reagent. Intended Use. Spirit Blue Agar is for use with Lipase Reagent or other lipid source for detecting and enumerating lipolytic microorganisms. Summary and Explanation. In 1941, Starr1 described a lipid emulsion medium for detecting.
Spirit Blue Agar Plate. What is this medium used for? Bacteria are identified based largely on what organic compounds they can break down. The range of compounds used depends on the collection of enzymes a species of bacteria can make. Lipids are organic compounds that some bacteria can use for carbon and
Suspend 32.15 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add 30 ml lipase substrate slowly while agitating to obtain an even distribution.
Adjusted as required to meet performance standards. Directions Suspend 37.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidise the methylene blue (i.e. restore its blue colour) and to suspend the
Tributyrin agar is prepared as an emulsion so that the agar will appear opaque. When the plate is inoculated with a lipase-positive organism, clear zones will appear around the growth as evidence of lipase activity. If no clear zones appear, the organism is lipase-negative (Figure 6-48). Spirit Blue Agar is also prepared as

Summary and Explanation. In 1941, Starr1 described a lipid emulsion medium for detecting lipolytic (lipase-producing) microorganisms to which he added the dye, spirit blue. Other dyes used as indicators of lipolysis were toxic to many microorganisms. Spirit blue did not have toxic effects. When testing samples of dairy
Prepared Media Plates are carefully tested for sterility and to verify their ability to support the characteristic growth of appropriate organisms. Unless you specify otherwise, we will ship prepared media plates on Monday, Tuesday, or Wednesday. When you receive your media, refrigerate immediately
With 3% Lipase Reagent – Pale blue, opalescent. Reaction of 3.5% Solution at 25°C: pH 6.8 ± 0.2. Difco™ Lipase Reagent Appearance: White, opaque emulsion. Cultural Response Difco™ Spirit Blue Agar and Lipase Reagent Prepare the medium per label directions. Inoculate and incubate at 35 ± 2°C for up to 72 hours.
Suspend 32.15 g of Spirit Blue Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and add slowly 30 ml of the lipase substrate while agitating to obtain an even distribution.
     

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